Philips, H1/H7 MasterDuty MaxiKit, Replacement Kit, 24 V

Product Information

Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact (see figures "RNeasy Mini Kit ACT environmental impact factor label US, EU and UK" The customer shall cooperate with VWR in all matters relating to the services, provide all such access and information as is necessary and obtain any licences permissions and consents required before commencement of the services. The PureLink™ HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. If the cells in RNAprotect Tissue Reagent cannot be collected by centrifugation, please try one of the following suggestions:

RNeasy Kits are the Gold standard for total RNA isolation. They provide fast purification of high-quality RNA from small to large amounts of cells, tissues, and yeast using silica-membrane RNeasy spin columns or 96-well plates. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system. The RNeasy 96 Kit enables high-throughput purification of total RNA from up to 96 cultured-cell samples using silica-membrane RNeasy 96 plates. A dedicated RNeasy QIAcube Kit enables automated purification of 1–12 samples on the QIAcube Connect. Find out which origin of replication your plasmid contains, andlook at the table below for classification into high-copy or low-copy types. This table can also be found online atthe QIAGEN Plasmid Resource Centerin the section' Growth of bacterial cultures; Plasmid Copy Number' .A way to determine experimentallyif the copy number of your plasmid is high or low is to perform a miniprep. A high-copy plasmid should yield between 3-5 ug DNA per 1 ml LB culture, while a low-copy plasmid will yield between 0.2-1 ug DNA per ml of LB culture. The dedicated RNeasy Mini QIAcube Kit, including rotor adapters preloaded with RNeasy spin columns and elution tubes, delivers greater convenience and time savings (see figure "Significant time savings with dedicated QIAcube Kits"). Authorisation to return products damaged during delivery must be requested within 3 days of delivery. VWR has the right to repair and return damaged products. Optionally, repeat the elution step, and incubate the spin column on the bench for 10 minutes with RNase-free water before centrifuging.

Which plasmid DNA maxiprep kit is right for you?

The customer is responsible for unloading and transporting large and/or heavy items from delivery vans and for supervising the unloading of all other products delivered.

An example of the calculations involved in RNA quantification is shown below. Use the buffer in which the RNA is diluted to zero the spectrophotometer:

ensure complete disruption and homogenization of the starting material as instructed in the section 'Disruption and homogenization of starting materials' of the handbook All intellectual property rights arising out of or in connection with the services shall be owned by VWR. Termination Authorisation will be subject to the condition that the products are returned to VWR Customer Service Centre or to the manufacturer or other source and by the method advised by VWR.

The QIAcube Connect uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into laboratory workflows. All steps in the purification procedure are fully automated — and up to 12 samples can be processed per run. The QIAcube Connect together with the dedicated RNeasy Mini QIAcube Kit provides fast, easy, and convenient RNA purification. Advertise or offer to sell or buy any goods or services for any business purpose, unless such Community Feature specifically allows such messages. Price on application’ (POA) quotations and all other quotations do not constitute offers and will be valid for 30 days from the date of the quotation, unless otherwise notified by VWR. Toensure efficient gDNA removal when doing an on-column digest using the RNase-Free DNase Set in combination with RNeasy Minithe following factors are crucial:

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VWR shall provide services to the customer in accordance with the specification agreed between them from time to time. Such services will be provided with all reasonable care and skill. Any liability accepted by VWR under this contract is in lieu of any terms implied by law as to the quality or fitness for any particular purpose of the products and/or the standard of the services and all such implied terms are, to the fullest extent permitted by law, excluded from the contract between VWR and the customer. The customer shall indemnify VWR against any claims made against VWR by the customer’s employees, contractors or agents. Intellectual property rights In addition, tissue/cell lysis steps are typically carried out with lysis buffers containing guanidine isothiocyanate, a potent protein denaturant. It is very important to use a sufficient amount of lysis buffer during RNA isolation. We recommend using at least 10x volume of lysis buffer to tissue/cell pellet.

strictly follow the protocol for on-column DNase Digestion in Appendix D of the RNeasy Mini Handbook (you can let wash buffer RW1 incubate on the column for 3-5 minutesbefore centrifuging to enhance removal of excess gDNA prior to applying the enzyme) Customers who exceed their credit limits will be asked to pay in advance for additional products and/or services until the account is settled. CompactPrep Plasmid Kits enable fast, large-scale plasmid purification. The use of a vacuum manifold allows up to 24 samples to be purified in parallel on a single workbench (see figure "Microcentrifuge column format") for plasmid midi and plasmid maxi preps. Up to 200 µg plasmid DNA is purified in less than 60 minutes from 25 ml (high-copy plasmids) or 50 ml (low-copy plasmids) LB medium using the CompactPrep Plasmid Midi Kit. DNA is eluted in 100 µl and concentrations are typically 1–2 µg/µl. The CompactPrep Plasmid Maxi Kit enables purification of up to 750 µg plasmid DNA in less than 60 minutes from 100 ml (high-copy plasmid) or 250 ml (low-copy plasmid) LB medium. DNA is eluted in 200 µl and concentrations are typically 3–4 µg/µl. CompactPrep Plasmid Kits provide molecular biology grade plasmid DNA with low endotoxin levels (see figure "Low endotoxin levels").Purity of RNA isolated with RNeasy Kits can be evaluated bydetermining the ratio ofabsorbance readingsat 260 nmand 280 nm (A260/A280). This ratio provides an estimate of the purity of RNA with respect to contaminants that absorb in the UV range, such as protein. In the rare case that trace amounts of genomic DNA are still detectable in sensitive downstream applications such as e.g.,realtime RT-PCR, an in-solution digest using the RNase-Free DNAase set can be performed. Instructionsare presentedin Appendix C of the RNeasy MinElute Cleanup Handbook. In view of the wide range of uses of chemicals and apparatus, the customer will be solely responsible for determining the suitability and specification of products, services, information and advice for its purposes. Publish, post, upload, distribute or disseminate any inappropriate, profane, defamatory, infringing, obscene, indecent or unlawful topic, name, material or information.